We have reported that transcription factor binding occurs in dense clusters. Since these clusters are enriched by known in vitro TF motifs, it is possible to predict the location of these clusters using motif matches. The accuracy of the prediction was found to be intimately related to the stringency level used. In order to optimise the stringency level for each TF, and in turn to make a better prediction for the location of the clusters, I hope to quantify the TFs in living cells and improve ChIP-Seq by using identical affinity tags for the different TFs.
Fangjie Zhu
